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使用 MALDI 质谱成像进行代谢分析的示例准备
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging
Please note that all translations are automatically generated.
Click here for the English version.
使用 MALDI 质谱成像进行代谢分析的示例准备
DOI:
10.3791/62008-v
•
09:08 min
•
December 22, 2020
•
Kelly Veerasammy*
1,2
,
Yuki X. Chen*
1,2
,
Sami Sauma*
1
,
Mathilde Pruvost
,
David K. Dansu
,
Tenzin Choetso
2
,
Tiffany Zhong
,
Damien Marechal
,
Patrizia Casaccia
,
Rinat Abzalimov
5
,
Ye He
5
1
The Graduate Center – Advanced Science Research Center, Neuroscience Initiative
,
The City University of New York
,
2
The City College of New York, CUNY
,
3
The Bronx High School of Science
,
4
The Graduate Center – Advanced Science Research Center, Structural Biology Initiative
,
The City University of New York
,
5
The Graduate Center – Advanced Science Research Center, MALDI MS Imaging Joint Core Facility
,
The City University of New York
Chapters
00:04
Introduction
00:46
Tissue Preparation
01:18
Cryosection Acquisition
03:24
Matrix Preparation
04:13
Matrix Deposition
06:16
Results: Representative Output with Selected m/z Spectra from Mass Spectrometry Acquired at 50-micron Lateral Resolution
07:37
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议的目标是在规划使用 MALDI MSI 进行实验时,为样品制备提供详细的指导,以最大限度地提高生物样品中的代谢和分子检测。
Tags
MALDI Imaging Mass Spectrometry
Metabolite Abundance And Distribution
Cryosectioning
Slide Preparation
MALDI Slide Conductivity
Sample Drying
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