JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
MALDI質量分析イメージングを用いた代謝プロファイリングのサンプル調製
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging
Please note that all translations are automatically generated.
Click here for the English version.
MALDI質量分析イメージングを用いた代謝プロファイリングのサンプル調製
DOI:
10.3791/62008-v
•
09:08 min
•
December 22, 2020
•
Kelly Veerasammy*
1,2
,
Yuki X. Chen*
1,2
,
Sami Sauma*
1
,
Mathilde Pruvost
,
David K. Dansu
,
Tenzin Choetso
2
,
Tiffany Zhong
,
Damien Marechal
,
Patrizia Casaccia
,
Rinat Abzalimov
5
,
Ye He
5
1
The Graduate Center – Advanced Science Research Center, Neuroscience Initiative
,
The City University of New York
,
2
The City College of New York, CUNY
,
3
The Bronx High School of Science
,
4
The Graduate Center – Advanced Science Research Center, Structural Biology Initiative
,
The City University of New York
,
5
The Graduate Center – Advanced Science Research Center, MALDI MS Imaging Joint Core Facility
,
The City University of New York
Chapters
00:04
Introduction
00:46
Tissue Preparation
01:18
Cryosection Acquisition
03:24
Matrix Preparation
04:13
Matrix Deposition
06:16
Results: Representative Output with Selected m/z Spectra from Mass Spectrometry Acquired at 50-micron Lateral Resolution
07:37
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
このプロトコルの目的は、生物学的サンプル中の代謝および分子検出を最大化するためにMALDI MSIを使用した実験を計画する際のサンプル調製に関する詳細なガイダンスを提供することです。
Tags
MALDI Imaging Mass Spectrometry
Metabolite Abundance And Distribution
Cryosectioning
Slide Preparation
MALDI Slide Conductivity
Sample Drying
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
MALDIサンプル調製:超薄層法
PAK2活性化のアミド水素/重水素交換とMALDI - TOF質量分析
同位体タグ質量分析法を用いたチオールレドックスプロテオームのプロファイリング
に基づいて、タンパク質 - タンパク質相互作用の同定のためのプロトコル<sup> 15</sup> Nの代謝標識、免疫沈降法、定量的質量分析法と親和性調節
に基づいた多タンパク質複合体の比較分析のための新たなアプローチ<sup> 15</sup> N代謝標識および定量的質量分析
単一のビリオン原子間力顕微鏡と超解像蛍光イメージングのサンプル調製
代謝産物の調査のためのMALDI-質量分析イメージング<em>ウマゴヤシtruncatula</em>ルート結節
質量サイトメトリー分析のための試料調製
ハイコンテンツ顕微鏡を用いた細胞レドックスプロファイリング
サンプル準備および透過電子顕微鏡によるエクソソームのイメージ投射
Read Article