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Prøveforberedelse til metabolisk profilering ved hjælp af MALDI Mass Spectrometry Imaging
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging
DOI:

09:08 min

December 22, 2020

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Chapters

  • 00:04Introduction
  • 00:46Tissue Preparation
  • 01:18Cryosection Acquisition
  • 03:24Matrix Preparation
  • 04:13Matrix Deposition
  • 06:16Results: Representative Output with Selected m/z Spectra from Mass Spectrometry Acquired at 50-micron Lateral Resolution
  • 07:37Conclusion

Summary

Automatic Translation

Målet med denne protokol er at give detaljeret vejledning om prøveforberedelsen, når der planlægges forsøg med MALDI MSI for at maksimere metabolisk og molekylær detektion i biologiske prøver.

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