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Journal
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Bioengineering
/
自组装血管化人体皮肤等价物的生成
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Generation of Self-assembled Vascularized Human Skin Equivalents
Please note that all translations are automatically generated.
Click here for the English version.
自组装血管化人体皮肤等价物的生成
DOI:
10.3791/62125-v
•
09:04 min
•
February 12, 2021
•
Martina M. Sanchez
,
Joshua T. Morgan
1
Departments of Bioengineering
,
University of California
Chapters
00:05
Introduction
00:42
Generation of 3D Collagen Dermal Component
01:44
Cell Laden Collagen Seeding of Dermal Compartment
03:16
Seeding of Epidermal Component and Stratification Induction
04:53
Immunofluorescent Staining of 3D Constructs
05:46
Prepare Imaging Wells
07:14
Results: Characterization of in Vitro Vascularized Human Skin Equivalents (VHSE)
08:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议的目标是使用可访问和简单的长期培养技术,描述血管化人类皮肤等价物的生成和体积分析。尽可能描述步骤的理由,使研究人员能够根据他们的研究需求定制。
Tags
Self-assembled
Vascularized
Human Skin Equivalents
Skin Model
Vasculature
Volumetric Imaging
Collagen
Cell Suspension
Air-liquid Interface
Cell Culture
Personalized Medicine
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