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Journal
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Immunology and Infection
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生产人类CRISPR工程的CAR-T细胞
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Production of Human CRISPR-Engineered CAR-T Cells
Please note that all translations are automatically generated.
Click here for the English version.
生产人类CRISPR工程的CAR-T细胞
DOI:
10.3791/62299-v
•
06:33 min
•
March 15, 2021
•
Sangya Agarwal*
1,2
,
Nils Wellhausen*
1,2
,
Bruce L. Levine
2
,
Carl H. June
2
1
Center for Cellular Immunotherapies, Perelman School of Medicine
,
University of Pennsylvania
,
2
Parker Institute for Cancer Immunotherapy
,
University of Pennsylvania
Chapters
00:05
Introduction
01:03
Designing of sgRNAs and Gene Disruption in Primary Human T-Cells
03:23
CRISPR Efficiency: Sequencing and Indel Detection
04:13
Results: Manufacturing Human CAR-T Cells Using CRISPR Cas9 Edited T-Cells
05:30
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们提出了一个协议,在原人类T细胞的基因编辑使用CRISPR卡斯技术来修改CAR-T细胞。
Tags
CRISPR-Cas9
CAR T Cell Therapy
Genome Editing
Human Clinical Trials
PBMC
CD4 T Cells
CD8 T Cells
IL-7
IL-15
Ribonucleoprotein (RNP) Complex
Electroporation
T-cell Activation And Expansion
Sequencing Primers
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