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여기에서는 말초혈액자연살해(PBNK), 간 조직으로부터 NK세포, 말초혈액단핵세포(PBMC) 또는 제대혈(CB)에서 유래한 키메라 항원 수용체(CAR)-NK세포를 확장시키는 방법을 제시한다. 이 프로토콜은 확장된 NK 세포의 최적화된 순도 외에도 221-mIL-21 영양세포를 사용하는 NK 및 CAR-NK 세포의 확장을 보여줍니다.