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JoVE Journal
Biochemistry

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Nano-differentialscanning fluormetri til screening i fragmentbaseret lead discovery
 
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Nano-differentialscanning fluormetri til screening i fragmentbaseret lead discovery

Article DOI: 10.3791/62469-v
May 16th, 2021

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Summary May 16th, 2021

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Click here for the English version.

Overvågning af ændringer i smeltetemperaturen af et målprotein (dvs.termisk skiftanalyse, TSA) er en effektiv metode til screening af fragmentbiblioteker af et par hundrede forbindelser. Vi præsenterer en TSA-protokol, der implementerer robotassisteret nanodifferenti differentialscanning af fluormetri (nano-DSF) til overvågning af iboende tryptofanlyscens og let rygspredning til fragmentscreening.

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