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Un metodo efficiente per l'induzione di cellule simili a epatociti diretti da cellule staminali embrionali umane
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells
DOI:

08:05 min

May 06, 2021

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Chapters

  • 00:04Introduction
  • 00:45Stem Cell Maintenance
  • 02:28Stem Cell Passage and Differentiation and Definitive Endoderm Formation
  • 03:46Differentiation of Hepatic Progenitor Cells and Hepatocyte Differentiation
  • 05:12Results: Directed Hepatocyte-like Cell Induction from Human Embryonic Stem Cells
  • 07:14Conclusion

Summary

Automatic Translation

Questo manoscritto descrive un protocollo dettagliato per la differenziazione delle cellule staminali embrionali umane (hESC) in cellule funzionali simili a epatociti (HHC) completando continuamente Activin A e CHIR99021 durante la differenziazione hESC in endoderma definitivo (DE).

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