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En effektiv metod för riktad hepatocytliknande cellinduktion från mänskliga embryonala stamceller
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells
DOI:

08:05 min

May 06, 2021

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Chapters

  • 00:04Introduction
  • 00:45Stem Cell Maintenance
  • 02:28Stem Cell Passage and Differentiation and Definitive Endoderm Formation
  • 03:46Differentiation of Hepatic Progenitor Cells and Hepatocyte Differentiation
  • 05:12Results: Directed Hepatocyte-like Cell Induction from Human Embryonic Stem Cells
  • 07:14Conclusion

Summary

Automatic Translation

Detta manuskript beskriver ett detaljerat protokoll för differentiering av mänskliga embryonala stamceller (hESCs) till funktionella hepatocytliknande celler (HLCs) genom att kontinuerligt komplettera Activin A och CHIR99021 under hESC-differentiering till slutgiltig endokderm (DE).

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