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Automatisering af aggregeret kvantificering i Caenorhabditis elegans
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Automatisering af aggregeret kvantificering i Caenorhabditis elegans

Article DOI: 10.3791/62997-v • 07:50 min • October 14th, 2021
October 14th, 2021 •
1, 1, 1, 1,2, 1, 1
1Department of Microbiology and Cell Science, University of Florida, 2Department of Computer and Information Science and Engineering, University of Florida

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Følgende protokol beskriver udviklingen og optimeringen af en arbejdsgang med høj kapacitet til ormedyrkning, fluorescensbilleddannelse og automatiseret billedbehandling for at kvantificere polyglutaminaggregater som en vurdering af ændringer i proteostase.

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Biologi udgave 176 C. elegans proteostase polyglutamin aggregering
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