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Drosophila melanogaster Larva Injection Protocol
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Immunology and Infection
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JoVE Journal Immunology and Infection
Drosophila melanogaster Larva Injection Protocol

Drosophila melanogaster Larva Injection Protocol

DOI:
10.3791/63144-v

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03:16 min

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October 19, 2021

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, ,
1Infection and Innate Immunity Laboratory, Department of Biological Sciences, Institute for Biomedical Sciences,The George Washington University

Chapters

  • 00:04Introduction
  • 00:36Larvae Selection for Infection
  • 00:57Injector Preparation
  • 01:28Larvae Injection
  • 02:20Results: Survival and Mortality Rate of Drosophila melanogaster
  • 02:42Conclusion

Summary

Automatic Translation

Automatic Translation

Drosophila melanogaster adult flies have been extensively utilized as model organisms to investigate the molecular mechanisms underlying host antimicrobial innate immune responses and microbial infection strategies. To promote the D. melanogaster larva stage as an additional or alternative model system, a larval injection technique is described.

Tags

Keywords: Drosophila MelanogasterLarvaInjectionProtocolMicroinjectionImmune StudiesMolecular StudiesRinger’s SolutionCapillaryMineral OilNanoliter InjectorTracheal SpiraclesPhotorhabdus AsymbioticaEscherichia ColiInnate ImmunityPathogen VirulenceAlternative Model

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