6,190 Views
•
03:16 min
•
October 19, 2021
DOI:
Summary
Automatically generated
Drosophila melanogaster adult flies have been extensively utilized as model organisms to investigate the molecular mechanisms underlying host antimicrobial innate immune responses and microbial infection strategies. To promote the D. melanogaster larva stage as an additional or alternative model system, a larval injection technique is described.
Related Videos
12:10
Protocols for Visualizing Steroidogenic Organs and Their Interactive Organs with Immunostaining in the Fruit Fly Drosophila melanogaster
Related Videos
10423 Views
12:16
Quantitative Measurement of the Immune Response and Sleep in Drosophila
Related Videos
15428 Views
09:25
Microinjection Techniques for Studying Mitosis in the Drosophila melanogaster Syncytial Embryo
Related Videos
14267 Views
05:32
Embryo Microinjection for Transgenesis in Drosophila
Related Videos
1909 Views
10:12
Systemic Bacterial Infection and Immune Defense Phenotypes in Drosophila Melanogaster
Related Videos
24652 Views
09:08
Assay for Blood-brain Barrier Integrity in Drosophila melanogaster
Related Videos
8252 Views
07:57
Imaging Intranuclear Actin Rods in Live Heat Stressed Drosophila Embryos
Related Videos
3439 Views
08:30
RNAi Interference by dsRNA Injection into Drosophila Embryos
Related Videos
16175 Views
05:52
Insulin Injection and Hemolymph Extraction to Measure Insulin Sensitivity in Adult Drosophila melanogaster
Related Videos
19728 Views
05:51
Microinjection of Live Drosophila Embryos: Early Delivery of Reagents to the Developing Embryo
Related Videos
4827 Views
Read Article
Cite this Article
Tafesh-Edwards, G., Kenney, E., Eleftherianos, I. Drosophila melanogaster Larva Injection Protocol. J. Vis. Exp. (176), e63144, doi:10.3791/63144 (2021).
Copy