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对小鼠皮肤中的中性粒细胞迁移进行成像，以研究生理条件下的亚细胞膜重塑
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Imaging Neutrophil Migration in the Mouse Skin to Investigate Subcellular Membrane Remodeling Under Physiological Conditions
Please note that all translations are automatically generated.
Click here for the English version.
对小鼠皮肤中的中性粒细胞迁移进行成像，以研究生理条件下的亚细胞膜重塑
DOI:
10.3791/63581-v
•
08:41 min
•
May 10, 2022
•
Nicolas Melis*
1
,
Bhagawat Subramanian*
1,2
,
Desu Chen
,
Roberto Weigert
1
Laboratory of Cellular and Molecular Biology, Center for Cancer Research
,
National Cancer Institute
,
2
Lineberger Comprehensive Cancer Center
,
UNC-Chapel Hill
Summary
Automatic Translation
English (Original)
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Automatic Translation
中性粒细胞迁移依赖于质膜在化学引诱剂及其与细胞外微环境的相互作用下快速和持续的重塑。本文描述的是一种基于活体亚细胞显微镜的程序，用于研究注射到麻醉小鼠耳朵中的中性粒细胞中膜重塑的动态。
Tags
Neutrophil Migration
Mouse Skin
Subcellular Membrane Remodeling
Intravital Subcellular Microscopy (ISMic)
Multiphoton Microscopy
Immune Cell Dynamics
Physiological Conditions
Adoptive Transfer
Motion Artifacts
Membrane Remodeling
Quantitative Analysis
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