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CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts
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CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

DOI:
10.3791/64209-v

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06:52 min

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September 13, 2022

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, , , , , , , , , ,
1Department of Biomedical Physiology and Kinesiology,Simon Fraser University

Chapters

  • 00:05Introduction
  • 00:42Design of CRISPR Components for Precise Edits
  • 02:56Reporter Gene Screening of CRISPR-Cas9-Edited Larval Zebrafish
  • 03:42Genotyping of CRISPR-Cas9-Edited Larval Zebrafish
  • 05:20Results: Validation of Gene-Edits and Analysis of Cardiac Consequences
  • 06:13Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes an approach to facilitate precise knock-in edits in zebrafish embryos using CRISPR-Cas9 technology. A phenotyping pipeline is presented to demonstrate the applicability of these techniques to model a Long QT Syndrome-associated gene variant.

Tags

Keywords: CRISPR-Cas9Knock-inZebrafishLong QT SyndromeSgRNA DesignHDR TemplateSanger SequencingPhenotyping

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