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CRISPR-Cas9-gemedieerde nauwkeurige knock-in bewerkingen in zebravisharten
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CRISPR-Cas9-gemedieerde nauwkeurige knock-in bewerkingen in zebravisharten

Article DOI: 10.3791/64209-v • 06:52 min • September 13th, 2022
September 13th, 2022 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Department of Biomedical Physiology and Kinesiology, Simon Fraser University

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Dit protocol beschrijft een aanpak om nauwkeurige knock-in bewerkingen in zebravisembryo's te vergemakkelijken met behulp van CRISPR-Cas9-technologie. Een fenotyperingspijplijn wordt gepresenteerd om de toepasbaarheid van deze technieken aan te tonen om een long QT-syndroom-geassocieerde genvariant te modelleren.

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Biologie Nummer 187 CRISPR-Cas9 zebravis homologie-gerichte reparatie hERG Long QT Syndroom
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