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ATAC-Seq Optimization for Cancer Epigenetics Research
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
ATAC-Seq Optimization for Cancer Epigenetics Research
DOI:

07:13 min

June 30, 2022

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Chapters

  • 00:04Introduction
  • 01:04Cell Lysis and Tn5 Tagmentation
  • 01:52DNA Purification
  • 02:57PCR Amplification
  • 03:43Beads Purification
  • 04:26Results: Comparison of TSS Enrichment Scores and Differential Peaks Obtained Using Optimized Cell‐Lysis Buffers, Cell Number Input, and Tn5 Concentration of ATAC‐Seq Libraries
  • 06:33Conclusion

Summary

Automatic Translation

ATAC-seq is a DNA sequencing method that uses the hyperactive mutant transposase, Tn5, to map changes in chromatin accessibility mediated by transcription factors. ATAC-seq enables the discovery of the molecular mechanisms underlying phenotypic alterations in cancer cells. This protocol outlines optimization procedures for ATAC-seq in epithelial cell types, including cancer cells.

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