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用于癌症表观遗传学研究的ATAC-Seq优化
JoVE Journal
Cancer Research
Author Produced
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JoVE Journal Cancer Research
ATAC-Seq Optimization for Cancer Epigenetics Research
DOI:

07:13 min

June 30, 2022

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Chapters

  • 00:04Introduction
  • 01:04Cell Lysis and Tn5 Tagmentation
  • 01:52DNA Purification
  • 02:57PCR Amplification
  • 03:43Beads Purification
  • 04:26Results: Comparison of TSS Enrichment Scores and Differential Peaks Obtained Using Optimized Cell‐Lysis Buffers, Cell Number Input, and Tn5 Concentration of ATAC‐Seq Libraries
  • 06:33Conclusion

Summary

Automatic Translation

ATAC-seq是一种DNA测序方法,它使用过度活跃的突变转座酶Tn5来绘制由转录因子介导的染色质可及性的变化。ATAC-seq能够发现癌细胞表型改变的分子机制。该协议概述了上皮细胞类型(包括癌细胞)中ATAC-seq的优化程序。

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