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Journal
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Biochemistry
/
聚合物拥挤脂质膜上的单分子扩散和组装
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes
Please note that all translations are automatically generated.
Click here for the English version.
聚合物拥挤脂质膜上的单分子扩散和组装
DOI:
10.3791/64243-v
•
10:43 min
•
July 19, 2022
•
Satyaghosh Maurya
,
Vishwesh Haricharan Rai
,
Aditya Upasani
,
Saurabh Umrao
2
,
Diksha Parwana
,
Rahul Roy
3
1
Department of Chemical Engineering
,
Indian Institute of Science
,
2
Holonyak Micro & Nanotechnology Lab
,
University of Illinois, Urbana-Champaign
,
3
Center for BioSystems Science and Engineering
,
Indian Institute of Science
Chapters
00:04
Introduction
00:53
Assembly of the Microfluidic Chamber
01:41
Making Supported Bilayers on Glass Substrate by Vesicle Fusion
04:22
Microscope Setup and Single-Particle Imaging Measurements
07:26
Image Acquisition for Counting Subunits in a Protein Assembly
08:40
Results: Analyzing the Binding of Cytolysin A (ClyA) Protein and Mobility of DNA Tracer
10:10
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,提出了一种使用单分子全内反射荧光(smTIRF)显微镜执行和分析单分子在人工拥挤脂质膜上的结合,迁移率和组装的协议。
Tags
Single-molecule Imaging
Polymer-crowded Lipid Membranes
Supported Lipid Bilayers
Membrane Biomolecular Reactions
Diffusion
Assembly
POPC
DOPE-PEG(2000)
Small Unilamellar Vesicles
Sonication
Microfluidic Imaging Chambers
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