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Biochemistry
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JoVE Journal Biochemistry
Difusión y ensamblaje de una sola molécula en membranas lipídicas llenas de polímeros
Preparation of High-Temperature Sample Grids for Cryo-EM
Journal (Biochemistry)
Preparation of High-Temperature Sample Grids for Cryo-EM
Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
Journal (Biochemistry)
Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
 
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Difusión y ensamblaje de una sola molécula en membranas lipídicas llenas de polímeros

Article DOI: 10.3791/64243-v • 10:43 min • July 19th, 2022
July 19th, 2022 •
1, 1, 1, 1,2, 1, 1,3
1Department of Chemical Engineering, Indian Institute of Science, 2Holonyak Micro & Nanotechnology Lab, University of Illinois, Urbana-Champaign, 3Center for BioSystems Science and Engineering, Indian Institute of Science

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Aquí, se presenta un protocolo para realizar y analizar la unión, movilidad y ensamblaje de moléculas individuales en membranas lipídicas artificiales apiñadas utilizando microscopía de fluorescencia de reflexión interna total de molécula única (smTIRF).

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Bioquímica Número 185
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