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Journal
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Immunology and Infection
/
初代アカゲザルB細胞の遺伝子編集
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Gene Editing of Primary Rhesus Macaque B Cells
Please note that all translations are automatically generated.
Click here for the English version.
初代アカゲザルB細胞の遺伝子編集
DOI:
10.3791/64858-v
•
09:53 min
•
February 10, 2023
•
Harald Hartweger
,
Rajeev Gautam
,
Yoshiaki Nishimura
,
Fabian Schmidt
5
,
Kai-Hui Yao
,
Amelia Escolano
6
,
Mila Jankovic
,
Malcolm A. Martin
,
Michel C. Nussenzweig
4
1
Laboratory of Molecular Immunology
,
The Rockefeller University
,
2
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases
,
National Institutes of Health
,
3
Laboratory of Retrovirology
,
The Rockefeller University
,
4
Howard Hughes Medical Institute
,
The Rockefeller University
,
5
Laboratory of Applied Virology and Precision Medicine
,
King Abdullah University of Science and Technology (KAUST)
,
6
Vaccine and Immunotherapy Center
,
Wistar Institute
Chapters
00:04
Introduction
00:48
Preparation and Culture of Rhesus Macaque B Cells
02:39
Primary Rhesus Macaque B Cell Gene Editing
07:36
Results: Genome Editing of Primary Rhesus Macaque B Cells
09:22
Conclusion
Summary
Automatic Translation
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Automatic Translation
B細胞療法の研究のために、CRISPR/Cas9および組換えアデノ随伴ウイルス血清型6を用いて初代アカゲザルB細胞を培養および遺伝子編集する方法を紹介します。
Tags
Gene Editing
Primary Rhesus Macaque B Cells
Cell Therapy
B Cell Culture
CRISPR-Cas9
RAAV6
Homology-directed Repair
Cell Activation
Cell Preparation
Electroporation
Transduction
Preclinical Research
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