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代骨髓衍生的双光子成像使用的小鼠树突状细胞

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JoVE Journal Biology

Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging

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代骨髓衍生的双光子成像使用的小鼠树突状细胞

DOI:

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13:13 min

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July 09, 2008

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Melanie P. Matheu, Debasish Sen, Michael D Cahalan, Ian Parker

¹Department of Physiology and Biophysics,University of California, Irvine (UCI), ²Department of Neurobiology and Behaviour,University of California, Irvine (UCI)

Chapters

00:05Title
01:42Bone marrow isolation and resuspension: Removing the femur
03:32Removal and re-suspension of the bone marrow.
06:21Incucyte
07:32Culturing of dendritic cells
11:26Collection of activated, antigen pulsed dendritic cells, intra dermal injection, and 2-photon imaging
12:47Conclusion

Summary

Automatic Translation

次级淋巴器官树突状细胞的抗原呈现启动T细胞介导的适应性免疫反应的关键。在这里，我们展示了文化的骨髓衍生的小鼠树突状细胞，活化，双光子成像标签。

Tags

Bone Marrow Derived Murine Dendritic Cells 2-photon Imaging Preparation Method CD11c High Dendritic Cells Culture Homing Draining Lymph Node Antigen Presentation T Cell Interaction Essen Instruments Incucyte Dendritic Cell Maturation Peripheral Lymph Nodes Antigen-specific Interactions Two-photon Imaging

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