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成像效应在耳朵上的记忆体T细胞诱导后收养的DTH
JoVE Journal
Biology
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JoVE Journal
Biology
Imaging Effector Memory T cells in the Ear After Induction of Adoptive DTH
Please note that all translations are automatically generated.
Click here for the English version.
成像效应在耳朵上的记忆体T细胞诱导后收养的DTH
DOI:
10.3791/907-v
•
09:59 min
•
August 14, 2008
•
Melanie P. Matheu
,
Christine Beeton
,
Ian Parker
,
K. George Chandy
,
Michael D. Cahalan
1
Department of Physiology and Biophysics
,
University of California, Irvine (UCI)
,
2
Department of Neurobiology and Behavior
,
University of California, Irvine (UCI)
Chapters
00:01
Title
00:07
Introduction
00:38
Induction of Adoptive DTH and Measuring Inflammation in the Ear
02:54
Removing Dermal Layers from Ear
05:27
Preparing Ear for 2-photon Imaging
07:48
Imaging the T cell Motility in the Ear
09:26
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们证明了诱导和记录一个迟发型超敏反应(DTH)反应在鼠耳进展的方法。这是由鼠耳组织编制的示范效应/记忆性T细胞反应的双光子成像。
Tags
Imaging
Effector Memory T Cells
Ear
Induction
Adoptive DTH
Delayed Type Hypersensitivity
Immune Reaction
CCR7-
T Lymphocytes
Method
Rat Ear
Two-photon Imaging
GFP-labeled
Ova-specific
Cell Line
Equilibrate
Challenge
Saline
Ova Conjugated To Texas-Red
Antigen-presenting Cells
Motility Of Cells
Deep Dermal Layer
Immune Response
Collagen Fibers
Second Harmonic Generation
Article
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