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Journal
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Biology
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Usando uma pinça Laser Para Manipulação neurônios isolados In Vitro
JoVE Journal
Biology
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JoVE Journal
Biology
Using Laser Tweezers For Manipulating Isolated Neurons In Vitro
Usando uma pinça Laser Para Manipulação neurônios isolados In Vitro
DOI:
10.3791/911-v
•
10:45 min
•
September 11, 2008
•
Robert Clarke
,
Jianfeng Wang
,
Ellen Townes-Anderson
1
Department of Neurology and Neuroscience, School of Medicine
,
University of Medicine and Dentistry of New Jersey – UMDNJ
Chapters
00:04
Intro
01:00
Fabrication of culture dishes with adhesive and non-adhesive surfaces
05:06
Preparation of retinal cells
07:34
Plating of cells on culture dishes
08:17
Laser tweezer manipulation
10:07
Observation of cell growth
10:45
Acknowledgements
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
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português (Portuguese)
русский (Russian)
español (Spanish)
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Automatic Translation
Este vídeo descreve a manipulação de neurônios cultivados usando laser pinças in vitro.
Tags
Laser Tweezers
Isolated Neurons
In Vitro
Retinal Neurons
Cell Processes
Regenerating Cell Processes
Retinal Cell Cultures
Dissection
Digestion
Trituration
Plating
Laser Tweezers Dishes
Sal-1 Antibodies
Poly-HEMA
Cell Repellant
Cell Adhesive
Laser-tweezers-controlled Micromanipulation
Cell Selection
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