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从人死后脑组织神经元细胞核分离
JoVE Journal
Biology
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JoVE Journal
Biology
Neuronal Nuclei Isolation from Human Postmortem Brain Tissue
Please note that all translations are automatically generated.
Click here for the English version.
从人死后脑组织神经元细胞核分离
DOI:
10.3791/914-v
•
10:58 min
•
October 01, 2008
•
Anouch Matevossian
,
Schahram Akbarian
1
Psychiatry, Brudnick Neuropsychiatric Research Institute
,
University of Massachusetts Medical School
Chapters
00:00
Title
00:56
Introduction
01:20
Extraction of Nuclei from Postmortem Human Brain
03:34
Immunostaining of Purified Nuclei
05:54
Isolation of Neuronal Nuclei by Fluorescence Activated Sorting
08:50
Concentration of Neuronal Nuclei by Centrifugation
10:08
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
脑组织细胞的异质性,构成一个明显的局限性染色,因为大多数分析缺乏单细胞分辨率的表观遗传标记的研究。通常是神经元与神经胶质细胞和其他非神经元细胞混合。我们提供了一个协议提取和收集从人脑的神经元细胞核。
Tags
Neuronal Nuclei Isolation
Neuronal Chromatin
Nuclear Organization
Chromatin And DNA-based Assays
Single Cell Resolution
Cellular Heterogeneity
Glia Cells
Non-neuronal Cells
Cell-type Specific Cultures
CNS Cells
Epigenetic Mechanisms
Hypotonic Lysis Buffer
Ultracentrifugation
Immunotagging
Anti-NeuN Antibody
Fluorescence-activated Sorting
Chromatin Immunoprecipitation Studies
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