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从人死后脑组织神经元细胞核分离
JoVE Journal
Biology
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JoVE Journal Biology
Neuronal Nuclei Isolation from Human Postmortem Brain Tissue
DOI:

10:58 min

October 01, 2008

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Chapters

  • 00:00Title
  • 00:56Introduction
  • 01:20Extraction of Nuclei from Postmortem Human Brain
  • 03:34Immunostaining of Purified Nuclei
  • 05:54Isolation of Neuronal Nuclei by Fluorescence Activated Sorting
  • 08:50Concentration of Neuronal Nuclei by Centrifugation
  • 10:08Conclusion

Summary

Automatic Translation

脑组织细胞的异质性,构成一个明显的局限性染色,因为大多数分析缺乏单细胞分辨率的表观遗传标记的研究。通常是神经元与神经胶质细胞和其他非神经元细胞混合。我们提供了一个协议提取和收集从人脑的神经元细胞核。

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