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使用CyDye DIGE Fluor公司最小染料的细胞表面蛋白的选择性标签
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Biology
Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes
Please note that all translations are automatically generated.
Click here for the English version.
使用CyDye DIGE Fluor公司最小染料的细胞表面蛋白的选择性标签
DOI:
10.3791/945-v
•
14:43 min
•
November 26, 2008
•
Asa Hagner-McWhirter
,
Maria Winkvist
,
Stephanie Bourin
,
Rita Marouga
1
Research and Development
,
GE Healthcare Bio-Sciences AB
Chapters
00:00
Title
00:10
Introduction to Cell Surface Protein Labeling with Ettan DIGE System
01:12
Overview of the Experiment
02:19
Sample Preparation
03:13
Labeling with CyDye DIGE Fluors
04:03
Incubation and Quenching
04:45
Cell Lysis
05:29
Rehydration of IPG Strips
06:31
First Dimension ISO-Electric Focusing
07:27
Gel Casting
07:55
Equilibration of IPG Strips
09:43
Second Dimension SDS-Page
10:29
Gel Scanning
10:52
Results of Cell Surface Protein Labeling
11:21
Comparison with Fractionated Runs
12:30
2D DIGE Analysis
13:27
Conclusions
Summary
Automatic Translation
English (Original)
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Automatic Translation
一个简单的和具体的方法是没有分馏步骤细胞表面蛋白的荧光标记和增强检测证明。差分在细胞表面的蛋白质丰度使用的二维(2 - D)电泳和Ettan™DIGE技术进行了分析。
Tags
Selective Labelling
Cell-surface Proteins
CyDye DIGE Fluor Minimal Dyes
Surface Proteins
Intracellular Signaling
Environmental Adaptation
Drug Treatment
Disease Pathogenesis
Pathology
Protein-protein Interactions
Sensitive Methods
Reliable Methods
Cell Surface Proteins Detection
2-D Electrophoresis
Biomarkers Detection
Complex Protein Samples
Differential Changes
Low Abundance Proteins
Fractionation
Enrichment
Hydrophobic Nature
High Molecular Weight Proteins
Intact Cells Protocol
Specific Labeling
CyDye DIGE Fluor Minimal Dyes
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