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Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
JoVE Journal
Biology
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JoVE Journal Biology
Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
DOI:

09:46 min

February 25, 2010

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Chapters

  • 00:00Title
  • 00:31Introduction
  • 01:05Preparing the pPICZaA Construct
  • 02:18Preparation of Electrocompetent Yeast Cells
  • 04:04Transformation by Electroporation
  • 06:06Protein Expression in Pichia pastoris
  • 08:42Results of Recombinant Protein Expression
  • 09:02Conclusion

Summary

Automatic Translation

The protocol describes protein expression using the methylotrophic yeast Pichia pastoris. The preparation of electrocompetent yeast cells, transformation of the vector with the gene of interest into P. pastoris and yeast DNA purification are also performed. Western blot analysis and protein purification build the last steps in this protein expression protocol.

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