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Isolation of Valvular Endothelial Cells
JoVE Journal
Biology
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JoVE Journal Biology
Isolation of Valvular Endothelial Cells
DOI:

11:04 min

December 29, 2010

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Chapters

  • 00:05Title
  • 01:13Introduction
  • 01:42Isolation of Heart Valve Leaflets
  • 03:03Isolation of Endothelial Layer
  • 05:22Isolation of Side Specific Endothelial Layer
  • 08:03Isolation of Interstitial Cells
  • 09:07Isolation of Valvular Endothelial Cells Representative Images
  • 10:30Conclusion

Summary

Automatic Translation

We provide a method for isolating and culturing pure populations of heart valve endothelial cells (VEC). VEC can be isolated from either side of the cusp or leaflet and immediately following, underlying interstitial cell (VIC) isolation is straightforward.

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