维持细胞培养基的Wolbachia

Biology

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Summary

本视频介绍了净化按蚊细胞系的Wolbachia pipientis,然后在无细胞培养基培养的共生细菌的方法。细菌的生存能力的检测证明。

Cite this Article

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Gamston, C., Rasgon, J. Maintaining Wolbachia in Cell-free Medium. J. Vis. Exp. (5), e223, doi:10.3791/223 (2007).

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Abstract

在这个视频协议,证明程序(1)净化的Wolbachia共生菌的培养蚊子细胞,(2)使用荧光检测,以确定纯化的Wolbachia的可行性及(3)保持在无细胞培养基外的Wolbachia 。纯净的Wolbachia仍然活着,但在细胞阶段不进行复制,直到进入真核细胞重新接种。外的Wolbachia纯化以这种方式将保持在室温下至少一个星期可行,可能更长。纯净的Wolbachia适合显微注射,DNA提取和其他应用。

Comments

1 Comment

  1. Dear sir
    I am interest of your work, Wolbachia, so i have some question that make me clear about culture bacteria endosymbionts as
    1. How did you infected Wolbachia in your medium?
    ². What's the strain of your laboratory wolbachia?
    Please forward to me if possible and i woud like to thank for your kind

    Best reguard

    Veerayuth Kittichai
    Entomology Unit
    Chulalongkorn University
    Thailand

    Reply
    Posted by: Anonymous
    September 15, 2010 - 10:00 PM

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