A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 00:37Introduction
- 01:02Preparation of Equipment and Dissection Area
- 03:12Dissection of the Hippocampus from a Postnatal Rat Pup
- 06:08Culturing Hippocampal Slices
- 07:10Examples of Hippocampal Slice Cultures
- 07:47Conclusion
We describe a method to prepare organotypic hippocampal slices that can be easily adapted to other brain regions. Brain slices are laid on porous membranes and culture media is allowed to form an interface. This method preserves the gross architecture of the hippocampus for up to 2 weeks in culture.
Tags
Organotypic Hippocampal Slice CulturesLimbic SystemLong-term MemorySpatial NavigationHippocampal NeuronsLong-term Potentiation (LTP)Learning And MemorySynaptic TransmissionSynaptic PlasticityGene ManipulationPharmacological InterventionSynaptic ProteinsHippocampal SlicesBrain RegionsViral InfectionBiolisticsBiochemical AssaysImagingElectrophysiological Experiments
