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Modeling and Imaging 3-Dimensional Collective Cell Invasion
JoVE Journal
Medicine
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JoVE Journal Medicine
Modeling and Imaging 3-Dimensional Collective Cell Invasion
DOI:

07:08 min

December 07, 2011

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Chapters

  • 00:05Title
  • 01:04Retroviral Labeling of Cells With Fluorescent Proteins
  • 02:45Inverse Matrigel Invasion Assay
  • 04:16Staining and Visualization
  • 06:07Contributions of Candidate Proteins to Collective Invasion
  • 06:50Conclusion

Summary

Automatic Translation

Models of tumor cell invasion into three-dimensional extracellular matrix better reflect the in vivo situation than two-dimensional motility assays. Using matrix invasion assays combined with confocal imaging of fluorescently-labeled cells, detailed information on invasion modes and the distinct contributions of leading versus following cells can be obtained.

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