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Rapid Fibroblast Removal from High Density Human Embryonic Stem Cell Cultures
JoVE Journal
Biology
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JoVE Journal Biology
Rapid Fibroblast Removal from High Density Human Embryonic Stem Cell Cultures
DOI:

07:12 min

October 28, 2012

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Chapters

  • 00:05Title
  • 01:34Preparation of Mouse Embryonic Fibroblasts (MEF) Feeders
  • 02:21Co-seeding hESCS onto MEFs
  • 03:55Depleting MEFs from the Co-culture
  • 04:50Results: hESCs Purified from Co-culture
  • 06:40Conclusion

Summary

Automatic Translation

Despite ongoing efforts to transition cultures to feeder-free conditions, the derivation and culture of human embryonic stem cells (hESC) remain largely dependent on co-cultures with mouse embryonic feeders (MEFs). Here, we show a novel methodology for rapidly removing feeders from hESC cultures prior to experimentation.

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