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Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells
JoVE Journal
Biology
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JoVE Journal Biology
Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells
DOI:

07:22 min

March 28, 2013

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Chapters

  • 00:05Title
  • 01:16Adipose Tissue Isolation
  • 02:13Tissue Cutting and Digestion
  • 03:09Preparing Cell Suspension
  • 03:58Plating Cells
  • 04:46Differentiate Cells
  • 05:47Results: Browning of Primary Adipocytes
  • 07:02Conclusion

Summary

Automatic Translation

Primary white preadipocytes isolated from white adipose tissues in mice can be differentiated into beige/brite cells. Presented here is a reliable cellular model system to study the molecular regulation of "browning" of white fat.

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