/
/
Enteric Bacterial Invasion Of Intestinal Epithelial Cells In Vitro Is Dramatically Enhanced Using a Vertical Diffusion Chamber Model
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:47Growth of Intestinal Epithelial Cell (IEC) Monolayers on 0.4 µm Filters
- 02:22Vertical Diffusion Chamber (VDC) Co-culture Assay Preparation
- 04:21Setting up of the VDC
- 06:14Disassembling of the VDC after Co-culture
- 08:34Results: The VDC Model Facilitates Greater Numbers of Bacterial-IEC Interactions and Invasions than Standard Cell Culture Assay Conditions
- 10:07Conclusion
Performing cell culture assays for investigating bacterial adhesion and invasion under aerobic conditions is usually unrepresentative of the in vivo environment. A Vertical Diffusion Chamber model allows study of interactions of the human pathogen Campylobacter jejuni with intestinal epithelial cells under more in vivo-like conditions, resulting in enhanced bacterial invasion.
