/
/
Culturing and Maintaining Clostridium difficile in an Anaerobic Environment
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:51Culturing, Enumerating, and Storing C. difficile from Stool Samples
- 05:59Culturing C. difficile from Frozen Glycerol Stocks
- 07:10Purifying Spores from C. difficile
- 09:12Results: C. difficile Successfully Grown on Various Media
- 10:16Conclusion
Clostridium difficile is a pathogenic bacterium that is a strict anaerobe and causes antibiotic associated diarrhea (AAD). Here, methods for isolating, culturing and maintaining C. difficile vegetative cells and spores are described. These techniques necessitate an anaerobic chamber, which requires regular maintenance to ensure proper conditions for optimal C. difficile cultivation.
