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Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor
JoVE Journal
Biology
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JoVE Journal Biology
Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor
DOI:

08:14 min

May 01, 2014

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Chapters

  • 00:05Title
  • 01:06Dissect Lower Hemi-mandibles from Adult Mouse
  • 02:09Incisors Isolation
  • 03:51Microdissection of the Labial CL
  • 05:31Primary Culture and Passage of Dental Epithelial Stem Cells
  • 06:55Results: Isolation and Culture of DESCs from the Adult Mouse Incisor
  • 07:47Conclusion

Summary

Automatic Translation

The continuously growing mouse incisor provides a model for studying renewal of dental tissues from dental epithelial stem cells (DESCs). A robust system for consistently and reliably obtaining these cells from the incisor and expanding them in vitro is reported here.

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