Préparation de l'échantillon pour l'unité Virion microscopie à force atomique et Super-résolution d'imagerie par fluorescence

1Department of Physics & Astronomy, University of Utah, 2Center for Cell and Genome, University of Utah
Published 1/02/2014


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Hodges, J. A., Saffarian, S. Sample Preparation for Single Virion Atomic Force Microscopy and Super-resolution Fluorescence Imaging. J. Vis. Exp. (83), e51366, doi:10.3791/51366 (2014).

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Name Company Catalog Number Comments
Glass coverslips (fPALM) Electron Microscopy Services 72225-01 25 mm Coverslips
PLL(20)-g[3.5]-PEG(2)/PEG(3.4)-Biotin (20%)  SuSoS - Stock: 0.5 mg/ml in PBS
NeutrAvidin biotin-binding protein Invitrogen A2666 Stock: 0.25 mg/ml in PBS
Alexa Fluor 647 goat anti-rabbit IgG (H+L)  Invitrogen A21245 1:200 in 0.2 M KPO4, 0.15 M NaCl, 10% Glycol, pH 7.2 buffer
α-VSV-G  Invitrogen ab34774 1:200 in 0.2 M KPO4, 0.15 M NaCl, 10% Glycol, pH 7.2 buffer
Gluox Sigma G2133-250KU Glucose oxidase type seven from Aspergillius 
Catalase Sigma C40-100mg Catalase from Bovine liver 
MEA Sigma 30070-10G Cysteamine (MEA
Stock Buffer 50 mM Tris-HCl (pH 8.0) + 10 mM NaCl + 10% glucose
NTE 10 mM Tris pH 7.4, 100 mM NaCl, 66 mM EDTA
Slide-A-Lyzer, mini dialysis unit Thermo Scientific 10,000 MW
Microscope Cover Glass: 35 CIRCLE #1 Fisherbrand 35 CIRCLE #1



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