A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:14Preparing Large Apple Juice Agar Plates
- 02:04Collecting Embryos and Pre-crosslinking Treatment
- 04:35In vivo Crosslinking
- 05:34Preparing Proteins and Immunoprecipitation
- 07:10Results: Crosslinked Tud Protein Complex Analysis
- 08:23Conclusion
Multi-component protein complexes play crucial roles during cellular function and development. Here we describe a method used to isolate native protein complexes from Drosophila embryos after in vivo crosslinking followed by purification of the crosslinked complexes for subsequent structure-function analysis.
