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Transient Expression of Proteins by Hydrodynamic Gene Delivery in Mice
JoVE Journal
Biology
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JoVE Journal Biology
Transient Expression of Proteins by Hydrodynamic Gene Delivery in Mice
DOI:

12:54 min

May 05, 2014

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Chapters

  • 00:05Title
  • 01:23Weighing Mice and Preparing Syringes
  • 03:38Hydrodynamic DNA Delivery
  • 08:18Large-volume Tail Vein Injection using an Alternative Hand Position
  • 09:41Representative Analysis of Transgene Expression
  • 11:50Conclusion

Summary

Automatic Translation

In vivo transfection of naked DNA by hydrodynamic gene delivery introduces genes into the tissue of an animal with minimal inflammatory response. Sufficient amounts of gene product are generated such that gene function and regulation as well as protein structure and function can be analyzed.

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