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Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions
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JoVE Journal Chemistry
Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions

Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions

DOI:
10.3791/51487-v

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13:00 min

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April 04, 2014

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, ,
1Laboratory of Bioinorganic Chemistry, Department of Pharmacy and Biotechnology,University of Bologna

Chapters

  • 00:05Title
  • 02:01Preparing Samples and Performing the Experiment
  • 06:47Data Analysis
  • 09:24Results: Quantification of Enzyme Catalysis by Isothermal Titration Calorimetry
  • 12:29Conclusion

Summary

Automatic Translation

Automatic Translation

Isothermal titration calorimetry measures heat flow released or absorbed in chemical reactions. This method can be used to quantify enzyme-catalysis. In this paper, the protocol for instrumental setup, experiment running, and data analysis is generally described, and applied to the characterization of enzymatic urea hydrolysis by jack bean urease.

Tags

Isothermal Titration CalorimetryITCEnzymatic ReactionsEnzyme KineticsThermodynamicsKcatKMEnthalpyUreaseJack Bean

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