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Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria
JoVE Journal
Biology
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JoVE Journal Biology
Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria
DOI:

11:53 min

September 18, 2014

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Chapters

  • 00:05Title
  • 01:54Isolation of Spinal Cord Mitochondria
  • 05:34Immunolabeling of Isolated Mitochondria for Flow Cytometry
  • 08:06Assaying Mitochondrial Transmembrane Potential and Superoxide Production by Flow Cytometry
  • 09:30Acquisition of Immunolabeled Isolated Mitochondria by Flow Cytometry
  • 10:21Results: Flow Cytometry Analysis of Isolated Mitochondria
  • 11:34Conclusion

Summary

Automatic Translation

Described here is a method to detect and quantify mitochondrial outer membrane proteins by immunolabeling of mitochondria isolated from rodent tissue and analysis by flow cytometry. This method can be extended to assess functional aspects of mitochondrial subpopulations.

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