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Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues
JoVE Journal
Biology
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JoVE Journal Biology
Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues
DOI:

12:07 min

November 22, 2014

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Chapters

  • 00:05Title
  • 02:26One-step DNA Labeling Using Aziridine Cofactors (SMIIing)
  • 05:07Two-step Protein Labeling Using Double-activated Cofactors (mTAG)
  • 08:41Results: Gel Analyses for SMILing and mTAG Reactions
  • 11:04Conclusion

Summary

Automatic Translation

DNA and proteins are sequence-specifically labeled with affinity or fluorescent reporter groups using DNA or protein methyltransferases and synthetic cofactor analogues. Depending on the cofactor specificity of the enzymes, aziridine or double activated cofactor analogues are employed for one- or two-step labeling.

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