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Intravenous Injections in Neonatal Mice
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JoVE Journal Biology
Intravenous Injections in Neonatal Mice

Intravenous Injections in Neonatal Mice

DOI:
10.3791/52037-v

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05:17 min

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November 11, 2014

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, ,
1Department of Neuroscience,Ohio State University, 2Center for Gene Therapy, Nationwide Children’s Hospital Research Institute,Ohio State University

Chapters

  • 00:05Title
  • 00:52Preparation and Injection Procedure
  • 03:06Post-Injection
  • 04:04Results: GFP Expression after Neonate Intravenous Injection
  • 04:55Conclusion

Summary

Automatic Translation

Automatic Translation

Animal models of pediatric disease can experience early onset and aggressive disease progression. Clinically relevant therapy delivery to young mouse models can be technically difficult. This protocol describes a non-invasive intravenous injection method for newborn mice within the first two postnatal days of life.

Tags

Intravenous InjectionNeonatal MiceTemporal VeinAdeno-associated VirusAAVTransgene ExpressionTherapeutic DeliveryMouse ModelsEarly Onset Disease

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