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Una strategia per convalidare il ruolo di callose mediata Plasmodesmal gating nella risposta Tropic
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Una strategia per convalidare il ruolo di callose mediata Plasmodesmal gating nella risposta Tropic

Article DOI: 10.3791/53513-v • 12:18 min • April 17th, 2016
April 17th, 2016 •
*1, *1, 1, 1, 2, 1
1Division of Applied Life Science (BK21 plus program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 2Biotechnology Research Institute, Chinese Academy of Agricultural Sciences
* These authors contributed equally

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Questo articolo descrive i metodi per lo screening dei geni che controllano la permeabilità plasmodesmal e pendenza, quindi, auxina durante la risposta tropico. Ciò comprende la misurazione del grado di risposta tropico in hypocotyl di Arabidopsis thaliana e controllando la permeabilità plasmodesmal da acido 8-idrossipirene-1,3,6-trisolfonico (HPTS) carico e valutazione del livello di fine callose.

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Biologia Cellulare Numero 110, Plasmodesmi (PD) callose auxina tropismo hypocotyl movimento symplasmic anilina blu acido 8-idrossipirene-1,3,6-trisolfonico (HPTS) mutante
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