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Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

DOI:
10.3791/56261-v

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09:33 min

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November 18, 2017

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, , , ,
1Department of Biological Sciences,University of Notre Dame

Chapters

  • 00:05Title
  • 00:50Embryo Fixation and Hybridization
  • 03:05Hot Washes, Blocking, Antibody Incubation, and Maleic Acid Buffer Washes
  • 04:27Probe Detection, Peroxidase Removal, and Immunofluorescence
  • 07:19Zebrafish Pronephros Mounting and Imaging
  • 08:23Results: Representative Zebrafish Pronephros and Multiciliated Cell (MCC) Imaging
  • 09:09Conclusion

Summary

Automatic Translation

Automatic Translation

Cilia development is vital to proper organogenesis. This protocol describes an optimized method to label and visualize ciliated cells of the zebrafish.

Tags

Multiciliated CellsZebrafishWhole Mount Fluorescent In Situ HybridizationImmunofluorescenceDevelopmental BiologyPronephrosCiliaRNA TranscriptsProtein LabelingAntibodiesFixationRehydrationProteinase KHybridization SolutionRNA Probe

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