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Journal
/
Biology
/
Broth Microdilution
In Vitro
Screening: An Easy and Fast Method to Detect New Antifungal Compounds
JoVE Journal
Biology
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JoVE Journal
Biology
Broth Microdilution
In Vitro
Screening: An Easy and Fast Method to Detect New Antifungal Compounds
Broth Microdilution
In Vitro
Screening: An Easy and Fast Method to Detect New Antifungal Compounds
DOI:
10.3791/57127-v
•
08:54 min
•
February 14, 2018
•
Calliandra Maria de-Souza-Silva
,
Fernanda Guilhelmelli
,
Daniel Zamith-Miranda
3
,
Marco Antônio de Oliveira
,
Joshua Daniel Nosanchuk
3
,
Ildinete Silva-Pereira*
1
,
Patrícia Albuquerque*
1,4
1
Laboratory of Molecular Biology, Department of Cellular Biology, Institute of Biological Sciences
,
University of Brasília
,
2
Department of Microbiology and Immunology
,
Albert Einstein College of Medicine
,
3
Division of Infectious Diseases, Department of Medicine
,
Albert Einstein College of Medicine
,
4
Faculty of Ceilândia
,
University of Brasília
Chapters
00:04
Title
00:47
Preparing Fungal Inoculum and Peptides
02:48
Antifungal Assay
05:23
Results: Detection of New Antimicrobial Peptides
07:10
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
An easy and adaptable broth microdilution method for screening antifungal compounds and extracts.
Tags
Broth Microdilution
In Vitro Screening
Antifungal Compounds
Fungal Activity
RPMI 1640 Medium
C. Albicans
C. Neoformans
Peptides
Serial Dilution
96-well Microplate
Article
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