Journal
/
/
Virus Delivery of CRISPR Guides to the Murine Prostate for Gene Alteration
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Virus Delivery of CRISPR Guides to the Murine Prostate for Gene Alteration
DOI:

06:21 min

April 27, 2018

, , , ,

Chapters

  • 00:04Title
  • 00:47Surgical Preparations
  • 01:40Virus Delivery to the Murine Prostate
  • 04:35Results: Loss of Pten Expression in the Prostate Using CRISPR/Cas9 Gene Editing
  • 05:45Conclusion

Summary

Automatic Translation

This protocol describes a newly established method for virus delivery to the murine prostate. Using either CRISPR/Cas9 technology, gene overexpression, or Cre recombinase delivery, the technique allows orthotopic alteration of gene expression and implements a novel mouse model for prostate cancer.

Related Videos

Read Article