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Virus Delivery of CRISPR Guides to the Murine Prostate for Gene Alteration
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Cancer Research
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JoVE Journal Cancer Research
Virus Delivery of CRISPR Guides to the Murine Prostate for Gene Alteration

Virus Delivery of CRISPR Guides to the Murine Prostate for Gene Alteration

DOI:
10.3791/57525-v

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06:21 min

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April 27, 2018

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, , , ,
1Department of Clinical Medicine,Aarhus University, 2GDD, Cancer Cell Biology Program,National Cancer Research Center (CNIO)

Chapters

  • 00:04Title
  • 00:47Surgical Preparations
  • 01:40Virus Delivery to the Murine Prostate
  • 04:35Results: Loss of Pten Expression in the Prostate Using CRISPR/Cas9 Gene Editing
  • 05:45Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a newly established method for virus delivery to the murine prostate. Using either CRISPR/Cas9 technology, gene overexpression, or Cre recombinase delivery, the technique allows orthotopic alteration of gene expression and implements a novel mouse model for prostate cancer.

Tags

CRISPRGene AlterationMurine ProstateVirus DeliveryProstate CancerIn VivoGene EditingSurgical ProcedureAnesthesiaAseptic TechniqueAbdominal IncisionSeminal VesicleInsulin SyringeGene Expression

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