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A Neurosphere Assay to Evaluate Endogenous Neural Stem Cell Activation in a Mouse Model of Minimal Spinal Cord Injury
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
A Neurosphere Assay to Evaluate Endogenous Neural Stem Cell Activation in a Mouse Model of Minimal Spinal Cord Injury
DOI:

09:08 min

September 13, 2018

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Chapters

  • 00:04Title
  • 00:45Minimal Spinal Cord Injury (SCI) Surgery
  • 04:01Spinal Cord Isolation and Neurosphere Culture
  • 07:18Results: Representative Neurosphere Analyses Following Minimal SCI
  • 08:24Conclusion

Summary

Automatic Translation

Here, we demonstrate the performance of a minimal spinal cord injury model in an adult mouse that spares the central canal niche housing endogenous neural stem cells (NSCs). We show how the neurosphere assay can be used to quantify activation and migration of definitive and primitive NSCs following injury.

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