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Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
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Bioengineering
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JoVE Journal Bioengineering
Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

DOI:
10.3791/58222-v

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10:28 min

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September 20, 2018

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Chapters

  • 00:04Title
  • 00:49Cell Line Culture for Lentivirus Production
  • 02:27Lentiviral Infection of Mouse Embryonic Stem Cells (mESCs)
  • 05:28Chemical Epigenetic Modifiers (CEM)s Preparation and Treatment
  • 06:47Microscopic and Flow Cytometry Analysis of Expression
  • 08:55Results: Effective Gene Repression Using Chemical Epigenetic Modifiers
  • 10:00Conclusion

Summary

Automatic Translation

Automatic Translation

Regulation of the chromatin environment is an essential process required for proper gene expression. Here, we describe a method for controlling gene expression through the recruitment of chromatin-modifying machinery in a gene-specific and reversible manner.

Tags

EpigeneticsGene TranscriptionChromatin RegulationChemical Epigenetic ModifiersGene Regulation MechanismsCell Culture293T HEK CellsMESCPolyethylenimine (PEI) TransfectionViral InfectionCell PassageTrypsinization

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