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ATAC-seq Assay with Low Mitochondrial DNA Contamination from Primary Human CD4+ T Lymphocytes
JoVE Journal
Genetics
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JoVE Journal Genetics
ATAC-seq Assay with Low Mitochondrial DNA Contamination from Primary Human CD4+ T Lymphocytes
DOI:

08:36 min

March 22, 2019

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Chapters

  • 00:04Title
  • 00:55Activation and Purification of CD4+ T Cells
  • 04:36Assay for Transposase-accessible Chromatin Sequencing (ATAC-seq)
  • 06:33Results: ATAC-seq and Sequencing Result Analysis
  • 07:48Conclusion

Summary

Automatic Translation

Here, we present a protocol to perform an assay for transposase-accessible chromatin sequencing (ATAC-seq) on activated CD4+ human lymphocytes. The protocol has been modified to minimize contaminating mitochondrial DNA reads from 50% to 3% through the introduction of a new lysis buffer.

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