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ATAC-seq Assay with Low Mitochondrial DNA Contamination from Primary Human CD4+ T Lymphocytes
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Genetics
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JoVE Journal Genetics
ATAC-seq Assay with Low Mitochondrial DNA Contamination from Primary Human CD4+ T Lymphocytes

ATAC-seq Assay with Low Mitochondrial DNA Contamination from Primary Human CD4+ T Lymphocytes

DOI:
10.3791/59120-v

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08:36 min

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March 22, 2019

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, ,
1Department of Biology,Boston University, 2Department of Computer Science and Engineering,University of California San Diego, 3Bioinformatics Program,Boston University

Chapters

  • 00:04Title
  • 00:55Activation and Purification of CD4+ T Cells
  • 04:36Assay for Transposase-accessible Chromatin Sequencing (ATAC-seq)
  • 06:33Results: ATAC-seq and Sequencing Result Analysis
  • 07:48Conclusion

Summary

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Here, we present a protocol to perform an assay for transposase-accessible chromatin sequencing (ATAC-seq) on activated CD4+ human lymphocytes. The protocol has been modified to minimize contaminating mitochondrial DNA reads from 50% to 3% through the introduction of a new lysis buffer.

Tags

ATAC-seqMitochondrial DNA ContaminationCD4+ T LymphocytesCell ActivationCell SelectionCell IsolationCell CountingMagnetic BeadsCell CultureCell Stimulation

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