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JoVE Journal Genetics
ATAC-seq ensaio com DNA mitocondrial baixa contaminação de primária humana CD4 linfócitos T
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ATAC-seq ensaio com DNA mitocondrial baixa contaminação de primária humana CD4 linfócitos T

Article DOI: 10.3791/59120-v • 08:36 min • March 22nd, 2019
March 22nd, 2019 •
1, 1,2, 1,3
1Department of Biology, Boston University, 2Department of Computer Science and Engineering, University of California San Diego, 3Bioinformatics Program, Boston University

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Aqui, apresentamos um protocolo para realizar um ensaio de cromatina transposase-acessível sequenciando (ATAC-seq) em linfócitos humanos registrados CD4 +. O protocolo foi modificado para minimizar contaminando leituras de DNA mitocondriais de 50% para 3%, através da introdução de um novo buffer de Lise.

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Genética edição 145 Epigenomics ATAC-seq Tn5 linfócitos CD4 + núcleos contaminação de DNA mitocondrial
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